![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4395014:Design
Designed by: Jiayuan Tian Group: iGEM22_SYSU-CHINA (2022-09-29)
SpyTag-AAs-yahK-SpyCatcher
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The idea of fusing SpyTag and SpyCatcher on the N-terminus and C-terminus, respectively, on the enzyme AAs and Yahk enables the fusion protein to catalyze efficiency in higher temperature than one where the reaction normally happens.
Source
The SpyTag/SpyCatcher system is a derivative of CnaB2 domain of Streptococcus pyogenes.AAs comes from the genome of Petroselinum crispum.Yahk comes from the genome of Escherichia coli.