Coding

Part:BBa_K4395009:Design

Designed by: Yaxuan Wu   Group: iGEM22_SYSU-CHINA   (2022-09-29)


SpyTag-vvGT2-SpyCatcher


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 66
    Illegal SpeI site found at 1014
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 66
    Illegal SpeI site found at 1014
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 66
    Illegal SpeI site found at 1014
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 66
    Illegal SpeI site found at 1014
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The idea of fusing SpyTag and SpyCatcher on the N-terminus and C-terminus, respectively, on the enzyme vvGT2 enables the fusion protein to catalyze glucosyl transfer in higher temperature than one where the reaction normally happens.


Source

The SpyTag/SpyCatcher system is a derivative of CnaB2 domain of Streptococcus pyogenes vvGT2 comes from Vitis vinifera.

References