Coding

Part:BBa_K4392010:Design

Designed by: Atharva Valanju   Group: iGEM22_IISER-Pune-India   (2022-10-11)


exoB gene from A.brasilense


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
    Illegal NgoMIV site found at 127
    Illegal NgoMIV site found at 131
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 538
    Illegal NgoMIV site found at 626
    Illegal NgoMIV site found at 866
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FP: TATATCTAGAATGACGGATCAAACGGCGGC (Tm=63.5 C; %GC=47) RP: TATATCTAGATCATGACCGGGAGGCGGACC (Tm=65.4 C; %GC=53)



Source

Derived from the genome of A. brasilense

References