Tag

Part:BBa_K4392002:Design

Designed by: Vikram Venugopal   Group: iGEM22_IISER-Pune-India   (2022-10-01)


6x Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the part belonged to the pHis17 plasmid backbone we used, we did not have to deal with any special design considerations.


Source

This part was sourced from the pHis17 plasmid backbone, onto which restriction-free cloning was done.

References