Part:BBa_K4392001:Design

ACC deaminase from Pseudomonas fluorescens

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 870
  Illegal XhoI site found at 187
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 975
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 210
  

Design Notes

We designed the part to include the BioBrick prefix and suffix so that there are enough restriction sites to facilitate simple cloning methods.

Source

The acdS gene from Pseudomonas fluorescens FY32 was obtained by us through gene synthesis.