Composite

Part:BBa_K4390114:Design

Designed by: Zhongyi Liang   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-10-09)


N-terminal L2NC-linker-tagged FAST-PETase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 647
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 876


Design Notes

The codon is optimized for JUMP assembly. There is no LacO in the parts sequence


Source

n

References