Composite
Part:BBa_K4388008:Design
Designed by: Tomas Lopes Group: iGEM22_KCL_UK (2022-10-09)
Transcriptional Unit 4 in Synthetic Operon for Pterostilbene Production
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 170
Illegal BamHI site found at 420 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For successful ligation in the correct order and orientation, the three basic parts (BBa_K4388011, BBa_K4388004, and BBa_B0015) were synthesised (IDT) with appropriate JUMP prefixes and suffixes, generating appropriate complementary 4-bp overhangs upon BsaI digestion.
Source
This part was created via JUMP type IIS assembly of basic parts BBa_K4388011, BBa_K4388004, and BBa_B0015 ligated together into pJUMP29-1D.