Coding

Part:BBa_K4388004:Design

Designed by: Tomas Lopes   Group: iGEM22_KCL_UK   (2022-10-05)


Vitis vinifera Resveratrol O-Methyltransferase Mutant (VvROMT S29P)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 116
    Illegal BamHI site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon-optimised for E.coli K12 strain, sequence had one PstI site which was removed to allow for RFC10 compatibility.


Source

Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6

Mutations added to wild-type sequence from GenBank code: FM178870.1.

References