Composite

Part:BBa_K4385019:Design

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-06)


Copper-inducible surface display


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 535
    Illegal XbaI site found at 28
    Illegal SpeI site found at 694
    Illegal PstI site found at 1042
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 535
    Illegal NheI site found at 1410
    Illegal NheI site found at 1433
    Illegal SpeI site found at 694
    Illegal PstI site found at 1042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 535
    Illegal BglII site found at 243
    Illegal BglII site found at 1545
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 535
    Illegal XbaI site found at 28
    Illegal SpeI site found at 694
    Illegal PstI site found at 1042
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 535
    Illegal XbaI site found at 28
    Illegal SpeI site found at 694
    Illegal PstI site found at 1042
    Illegal AgeI site found at 361
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is mainly composed of Copper-inducible promoter PcusC(BBa_K4385008), the anchoring protein OmpC-CBP(BBa_K4385003, BBa_K4385005) and heavy metal detoxification protein( BBa_K4385010).



Source

The basic parts that make up the composite part are all derived from E. coli genomic DNA.

References