Composite

Part:BBa_K4385018

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-06)


Lead-inducible surface display

The part mainly consists of the lead-inducible promoter pbr , the anchoring protein Lpp-OmpA and the heavy metal detoxification protein metallothionein. When the lead ion concentration in the environment exceeds a certain concentration, the pbr promoter initiates transcription of downstream OmpC-CBP, witch is used as an anchoring protein to anchor PbrR on the surface of E. coli for lead ion adsorption.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1627
    Illegal PstI site found at 462
    Illegal PstI site found at 1569
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1809
    Illegal NheI site found at 1832
    Illegal SpeI site found at 1627
    Illegal PstI site found at 462
    Illegal PstI site found at 1569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1944
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1627
    Illegal PstI site found at 462
    Illegal PstI site found at 1569
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1627
    Illegal PstI site found at 462
    Illegal PstI site found at 1569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI site found at 1360


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Categories
Parameters
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