Composite

Part:BBa_K4385010:Design

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-01)


Heavy metal detoxification protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It consists of a constitutive promoter J23100 and metallothionein BBa_K4385006, which can improve the tolerance of engineered bacteria to a variety of heavy metals when added to heavy metal surface display parts.


Source

It is amplified from E. coli genomic DNA.

References