![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4385010:Design
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-01)
Heavy metal detoxification protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 142
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It consists of a constitutive promoter J23100 and metallothionein BBa_K4385006, which can improve the tolerance of engineered bacteria to a variety of heavy metals when added to heavy metal surface display parts.
Source
It is amplified from E. coli genomic DNA.