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Coding
Part:BBa_K4384004
Designed by: Zike Yao Group: iGEM22_HK_SSC (2022-10-09)
5YSS_WT
This cyclase is expected to convert the molecule 5AVA to valerolactam. It is the original cyclase. We have chosen to use this cyclase as it was reported that an ORF encoding CF3HBD from Citrobacter freundii (CF3HBD) has a positive fluorescence signal corresponding to the conversion of 6-ACA to ε-caprolactam, which indicates that it may also bind to 5AVA and convert to valerolactam [1].
References: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 10
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |