Coding

Part:BBa_K4384002

Designed by: Zike Yao   Group: iGEM22_HK_SSC   (2022-10-09)


nitR_L117W

This protein is expected to bind to valerolactam. It is modified by our team by mutating the 117th residue leucine to tryptophan. We have modified the protein as it was reported that L117W is a mutant of nitR which is the second most sensitive to ε-caprolactam. This protein is found present in Alcaligenes faecalis. [1]

Reference: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None