Composite

Part:BBa_K4383062:Design

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-10-12)


J23101-EryO-B0034-TMADH-his operon terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 536
    Illegal PstI site found at 331
    Illegal PstI site found at 1930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 536
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 331
    Illegal PstI site found at 1930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 536
    Illegal XhoI site found at 1865
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 536
    Illegal PstI site found at 331
    Illegal PstI site found at 1930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 536
    Illegal PstI site found at 331
    Illegal PstI site found at 1930
    Illegal AgeI site found at 1027
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For more information, please view https://2022.igem.wiki/scu-china/design.html.TMADH is a gift from our partnership HZAU-China

Source

Brucella abortus


References