Coding
PqqF

Part:BBa_K4383024:Design

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


PqqF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 40
    Illegal PstI site found at 328
    Illegal PstI site found at 520
    Illegal PstI site found at 583
    Illegal PstI site found at 703
    Illegal PstI site found at 955
    Illegal PstI site found at 1117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 40
    Illegal PstI site found at 328
    Illegal PstI site found at 520
    Illegal PstI site found at 583
    Illegal PstI site found at 703
    Illegal PstI site found at 955
    Illegal PstI site found at 1117
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 40
    Illegal PstI site found at 328
    Illegal PstI site found at 520
    Illegal PstI site found at 583
    Illegal PstI site found at 703
    Illegal PstI site found at 955
    Illegal PstI site found at 1117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 40
    Illegal PstI site found at 328
    Illegal PstI site found at 520
    Illegal PstI site found at 583
    Illegal PstI site found at 703
    Illegal PstI site found at 955
    Illegal PstI site found at 1117
    Illegal NgoMIV site found at 103
    Illegal NgoMIV site found at 666
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 1675
    Illegal NgoMIV site found at 2243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 714
    Illegal SapI.rc site found at 1330


Design Notes

For more information, please view https://2022.igem.wiki/scu-china/design.html.

Source

Klebsiella pneumoniae

References

Zhu W, Klinman JP. Biogenesis of the peptide-derived redox cofactor pyrroloquinoline quinone. Curr Opin Chem Biol. 2020;59:93-103. doi:10.1016/j.cbpa.2020.05.001