Coding
PqqE

Part:BBa_K4383023:Design

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


PqqE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 70
    Illegal PstI site found at 1062
    Illegal PstI site found at 1087
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 70
    Illegal PstI site found at 1062
    Illegal PstI site found at 1087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 219
    Illegal XhoI site found at 1039
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 70
    Illegal PstI site found at 1062
    Illegal PstI site found at 1087
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 70
    Illegal PstI site found at 1062
    Illegal PstI site found at 1087
    Illegal NgoMIV site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 715


Design Notes

For more information, please view https://2022.igem.wiki/scu-china/design.html.

Source

Klebsiella pneumoniae

References

Zhu W, Klinman JP. Biogenesis of the peptide-derived redox cofactor pyrroloquinoline quinone. Curr Opin Chem Biol. 2020;59:93-103. doi:10.1016/j.cbpa.2020.05.001