Coding
PqqC

Part:BBa_K4383021:Design

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


PqqC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 423
    Illegal PstI site found at 631
    Illegal PstI site found at 691
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 423
    Illegal PstI site found at 631
    Illegal PstI site found at 691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 423
    Illegal PstI site found at 631
    Illegal PstI site found at 691
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 423
    Illegal PstI site found at 631
    Illegal PstI site found at 691
    Illegal AgeI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For more information, please view https://2022.igem.wiki/scu-china/design.html.

Source

Klebsiella pneumoniae

References

Bonnot F, Iavarone AT, Klinman JP. Multistep, eight-electron oxidation catalyzed by the cofactorless oxidase, PqqC: identification of chemical intermediates and their dependence on molecular oxygen [published correction appears in Biochemistry. 2013 Aug 20;52(33)5704]. Biochemistry. 2013;52(27):4667-4675. doi:10.1021/bi4003315