Coding
SldH

Part:BBa_K4383016:Design

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-09-30)


SldH(polyol dehydrogenase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1218
    Illegal NheI site found at 2439
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1218
    Illegal BglII site found at 1674
    Illegal BamHI site found at 1694
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1218
    Illegal NgoMIV site found at 809
    Illegal NgoMIV site found at 2214
    Illegal AgeI site found at 238
    Illegal AgeI site found at 1406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1182
    Illegal BsaI.rc site found at 1735
    Illegal SapI.rc site found at 745


Design Notes

sldH was used for oxidizing erythritol to L-erythrulose.For more information, please view https://2022.igem.wiki/scu-china/design.html.

Source

Gluconobacter suboxydans IFO 3255

References

Hoshino T, Sugisawa T, Shinjoh M, Tomiyama N, Miyazaki T. Membrane-bound D-sorbitol dehydrogenase of Gluconobacter suboxydans IFO 3255--enzymatic and genetic characterization. Biochim Biophys Acta. 2003 Apr 11;1647(1-2):278-88. doi: 10.1016/s1570-9639(03)00071-2.