Translational_Unit

Part:BBa_K4382014:Design

Designed by: UZAIR MOTIWALA   Group: iGEM22_IISER_Bhopal   (2022-10-12)


Pectinase gene construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2752
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 690
    Illegal BamHI site found at 2896
    Illegal BamHI site found at 3784
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2752
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2752
    Illegal NgoMIV site found at 3047
    Illegal AgeI site found at 2903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 794
    Illegal BsaI site found at 1168
    Illegal BsaI site found at 1273
    Illegal BsaI site found at 2242


Design Notes

The gene construct was codon optimized for Bacillus Subtilis


Source

The gene construct was synthesized from IDT

References