Coding

Part:BBa_K4378002:Design

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)

MHETase from I. sakaiensis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
    Illegal BglII site found at 1667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 351
    Illegal AgeI site found at 1089
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

During the design of this part, two internal PstI sites were mutated out in silico prior to synthesis. Additionally this part has been codon optimized for E. coli.

Source

Sequence originates from Ideonella sakaiensis, the sequence published here was adapted from the following publication:

Knott BC, Erickson E, Allen MD, Gado JE, Graham R, Kearns FL, Pardo I, Topuzlu E, Anderson JJ, Austin HP, Dominick G, Johnson CW, Rorrer NA, Szostkiewicz CJ, Copié V, Payne CM, Woodcock HL, Donohoe BS, Beckham GT, McGeehan JE. 2020. Characterization and engineering of a two-enzyme system for plastics depolymerization. Proceedings of the National Academy of Sciences 117: 25476–25485.