Part:BBa_K4377009:Design
Y167 mRFP Y240
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 711
Illegal AgeI site found at 823 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed mRFP(Part:E1010) as the functional protein between the two peptide regions(Part:K4377001 and Part:K4377002), in an attempt to verify the feasibility of self-assembling. ( result in Figure 1.) We choose mRFP as functional protein for testing because mRFP is easy to observe and check protein’s function.In order to purify our protein after expression, we added 10x His tag at the N terminal of Ubx protein. The reason we add 10x His tag at N terminal but not C terminal is to amplify the production of Ubx protein. To improve performance, we performed codon harmonization. The optimized sequence of codons will be more suitable for expression by our engineered E.coli.
Build
Source
Drosophila melanogaster, sequence found through Uniprot https://www.uniprot.org/uniprotkb/P83949/entry