Composite

Part:BBa_K4377009:Design

Designed by: Cheng Ching Hung   Group: iGEM22_NYCU_Formosa   (2022-10-13)


Y167 mRFP Y240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 711
    Illegal AgeI site found at 823
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed mRFP(Part:E1010) as the functional protein between the two peptide regions(Part:K4377001 and Part:K4377002), in an attempt to verify the feasibility of self-assembling. ( result in Figure 1.) We choose mRFP as functional protein for testing because mRFP is easy to observe and check protein’s function.In order to purify our protein after expression, we added 10x His tag at the N terminal of Ubx protein. The reason we add 10x His tag at N terminal but not C terminal is to amplify the production of Ubx protein. To improve performance, we performed codon harmonization. The optimized sequence of codons will be more suitable for expression by our engineered E.coli.

Build

Source

Drosophila melanogaster, sequence found through Uniprot https://www.uniprot.org/uniprotkb/P83949/entry

References