DNA

Part:BBa_K4369004

Designed by: Alexa Grebel   Group: iGEM22_Duesseldorf   (2022-10-11)


Cellulose synthase acsAB - Improvment of part BBa_K1321334

This part is an improvement of BBa_K1321334. Just a His-Tag was added. Acs A and Acs B are subunits in the cellulose operon of Komagataeibacter xylinus. The cellulose synthase consists of two domains, the catalytic domain (AcsA) and the regulatory domain (AcsB). AcsA catalyzes the polymerization of UDP-glucose to glucose and AcsB binds c-di-GMP which starts the polymerization process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1831
    Illegal BglII site found at 2545
    Illegal BglII site found at 4468
    Illegal BamHI site found at 841
    Illegal BamHI site found at 3835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1817
    Illegal AgeI site found at 1894
    Illegal AgeI site found at 2450
    Illegal AgeI site found at 3251
    Illegal AgeI site found at 3343
    Illegal AgeI site found at 3449
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None