DNA

Part:BBa_K4369000:Design

Designed by: Merve Seker   Group: iGEM22_Duesseldorf   (2022-10-11)


alg8 is the catalytic subunit of the alginate polymerase complex.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 650
    Illegal EcoRI site found at 805
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 650
    Illegal EcoRI site found at 805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 650
    Illegal EcoRI site found at 805
    Illegal BglII site found at 615
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 650
    Illegal EcoRI site found at 805
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 650
    Illegal EcoRI site found at 805
    Illegal NgoMIV site found at 469
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The genome of Azotobacter vinelandii for example shows a high GC content, which can be a problem in the synthesis. Future teams should consider finding a matching polymerase since the commonly used T7 promoter is not compatible. Since the fragment is quite long a two part amplification would be suitable.


Source

Genomic sequence of alg8 and alg44 genes in Azotobacter vinelandii

https://www.ncbi.nlm.nih.gov/nuccore/Y08819.1


References