Coding

Part:BBa_K4367015:Design

Designed by: Anders Kllberg   Group: iGEM22_Chalmers-Gothenburg   (2022-10-11)


PYL-nTEV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 601
    Illegal BamHI site found at 961
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 968


Design Notes

The part has been designed to allow for the golden gate assembley MoClo, and as such contains MoClo overhangs for MoClo implementation. A 6xHis-tag is included for extraction of the protein.


Source

PYL is a receptor for abscisic acid in plants. TEVp is derived from Tobacco Etch Virus protease.

References