Coding

Part:BBa_K4367002:Design

Designed by: August Qvist   Group: iGEM22_Chalmers-Gothenburg   (2022-08-01)


vioC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 547
    Illegal PstI site found at 1234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 547
    Illegal PstI site found at 1234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 547
    Illegal BglII site found at 114
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 547
    Illegal PstI site found at 1234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 547
    Illegal PstI site found at 1234
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for Saccharomyces cerevisiae. Overhangs for making the part moClo compatible as a type 3 moClo part.


Source

This part has been codon optimized for saccharomyces cerevisiae and ordered as synthetic DNA. The original gene is found in the Chromobacterium violaceum genome. DNA sequence converted from peptide sequence found through Genbank: https://www.ncbi.nlm.nih.gov/protein/AAD51810.1

References