Plasmid

Part:BBa_K4362023:Design

Designed by: Liuziteng Wang   Group: iGEM22_Tianjin   (2022-09-08)


42H-gal-dCas9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4223
    Illegal EcoRI site found at 5902
    Illegal EcoRI site found at 6131
    Illegal EcoRI site found at 11134
    Illegal XbaI site found at 607
    Illegal PstI site found at 11044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4223
    Illegal EcoRI site found at 5902
    Illegal EcoRI site found at 6131
    Illegal EcoRI site found at 11134
    Illegal PstI site found at 11044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4223
    Illegal EcoRI site found at 5902
    Illegal EcoRI site found at 6131
    Illegal EcoRI site found at 11134
    Illegal BglII site found at 4508
    Illegal BglII site found at 4762
    Illegal BglII site found at 7366
    Illegal BamHI site found at 8169
    Illegal XhoI site found at 9199
    Illegal XhoI site found at 10350
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4223
    Illegal EcoRI site found at 5902
    Illegal EcoRI site found at 6131
    Illegal EcoRI site found at 11134
    Illegal XbaI site found at 607
    Illegal PstI site found at 11044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4223
    Illegal EcoRI site found at 5902
    Illegal EcoRI site found at 6131
    Illegal EcoRI site found at 11134
    Illegal XbaI site found at 607
    Illegal PstI site found at 11044
    Illegal NgoMIV site found at 9741
    Illegal AgeI site found at 3826
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2437
    Illegal BsaI site found at 4481
    Illegal SapI.rc site found at 3519
    Illegal SapI.rc site found at 4334
    Illegal SapI.rc site found at 7665


Design Notes

This optimized sequence is used in Saccharomyces cerevisiae


Source

This sequence is optimized to be used in Saccharomyces cerevisiae

References