Composite

Part:BBa_K4362022:Design

Designed by: Liuziteng Wang   Group: iGEM22_Tianjin   (2022-09-08)


PmCDA1-dCas9-UGI with NLS label


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 23
    Illegal EcoRI site found at 1702
    Illegal EcoRI site found at 1931
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 23
    Illegal EcoRI site found at 1702
    Illegal EcoRI site found at 1931
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 23
    Illegal EcoRI site found at 1702
    Illegal EcoRI site found at 1931
    Illegal BglII site found at 308
    Illegal BglII site found at 562
    Illegal BglII site found at 3166
    Illegal BamHI site found at 3969
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 23
    Illegal EcoRI site found at 1702
    Illegal EcoRI site found at 1931
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 23
    Illegal EcoRI site found at 1702
    Illegal EcoRI site found at 1931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 281
    Illegal SapI.rc site found at 134
    Illegal SapI.rc site found at 3465


Design Notes

This optimized sequence is used in Saccharomyces cerevisiae


Source

This sequence is optimized to be used in Saccharomyces cerevisiae

References