Composite

Part:BBa_K4362018:Design

Designed by: Liuziteng Wang   Group: iGEM22_Tianjin   (2022-09-08)


Micro Venus with homologous arms


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3327
    Illegal XbaI site found at 3445
    Illegal SpeI site found at 506
    Illegal SpeI site found at 2780
    Illegal SpeI site found at 5250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3327
    Illegal NheI site found at 1640
    Illegal NheI site found at 2538
    Illegal SpeI site found at 506
    Illegal SpeI site found at 2780
    Illegal SpeI site found at 5250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3327
    Illegal BglII site found at 493
    Illegal BglII site found at 3448
    Illegal BglII site found at 3543
    Illegal BamHI site found at 5747
    Illegal XhoI site found at 1
    Illegal XhoI site found at 685
    Illegal XhoI site found at 1455
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3327
    Illegal XbaI site found at 3445
    Illegal SpeI site found at 506
    Illegal SpeI site found at 2780
    Illegal SpeI site found at 5250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3327
    Illegal XbaI site found at 3445
    Illegal SpeI site found at 506
    Illegal SpeI site found at 2780
    Illegal SpeI site found at 5250
    Illegal AgeI site found at 4643
    Illegal AgeI site found at 5030
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5699


Design Notes

This sequence is a code based on our secondary model


Source

From our seecondary coding model

References