Coding

Part:BBa_K4362005:Design

Designed by: Wang   Group: iGEM22_Tianjin   (2022-09-06)


dCas9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1337
    Illegal BglII site found at 2572
    Illegal BamHI site found at 3375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1337
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2871


Design Notes

expression in S. cerevisiae


Source

From Target-AID (CBE)

References