Coding

Part:BBa_K4359009:Design

Designed by: Ira Zibbu   Group: iGEM22_IISER_TVM   (2022-10-07)


ClyA-3XFLAG-THP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 925
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 925
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 925
    Illegal BglII site found at 81
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 925
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was designed with an EcoN1 site upstream and BamH1 site downstream to enable ligation into the E.coli expression vector pGEX-4T1. Additional nucleotides flanking the restriction site were included to ensure efficient digestions by restriction enzymes. The sequence on the registry only includes the CDS.


Source

This part contains fragments from the E.coli genome (BBa_K4359001), as well as engineered versions of natural proteins (BBa_K4359003), (BBa_K4359007) and (BBa_K4359002).

References