Coding

Part:BBa_K4359004:Design

Designed by: Ira Zibbu   Group: iGEM22_IISER_TVM   (2022-09-02)


Tumour homing peptide THP Pep-1 against SKBR3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was designed for optimized production in an E.coli chassis. The sequence was also made amenable to gene synthesis by utilizing the Integrated DNA Technologies (IDT) codon optimization tool.


Source

The protein sequence for this part is adapted from reference [2].

References

[1] Kapoor, P., Singh, H., Gautam, A., Chaudhary, K., Kumar, R., & Raghava, G. P. S. (2012). TumorHoPe: A Database of Tumor Homing Peptides. PLoS ONE, 7(4), e35187. https://doi.org/10.1371/journal.pone.0035187

[2] Pereira, A. C., Ferreira, D., Santos‐Pereira, C., Vieira, T. F., Sousa, S. F., Sales, G., & Rodrigues, L. R. (2021). Selection of a new peptide homing SK‐BR‐3 breast cancer cells. Chemical Biology & Drug Design, 97(4), 893–903. https://doi.org/10.1111/cbdd.13816

[3] Kondo, E., Iioka, H., & Saito, K. (2021). Tumor‐homing peptide and its utility for advanced cancer medicine. Cancer Science, 112(6), 2118–2125. https://doi.org/10.1111/cas.14909