Plasmid

Part:BBa_K4357006:Design

Designed by: Di Yu   Group: iGEM22_UM_Macau   (2022-10-12)


J23100-mCherry-pS1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3005
    Illegal suffix found in sequence at 957
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3005
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 958
    Illegal PstI site found at 972
    Illegal NotI site found at 776
    Illegal NotI site found at 965
    Illegal NotI site found at 3011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3005
    Illegal XhoI site found at 785
    Illegal XhoI site found at 1989
    Illegal XhoI site found at 2881
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3005
    Illegal suffix found in sequence at 958
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3005
    Illegal XbaI site found at 3020
    Illegal SpeI site found at 958
    Illegal PstI site found at 972
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The J23100 consituitive promoter was usssd to control the mCherry signaling protein


Source

https://parts.igem.org/Part:BBa_K4357003 https://parts.igem.org/Part:BBa_B0034 https://parts.igem.org/Part:pSB1C3 https://parts.igem.org/Part:BBa_B0015 https://parts.igem.org/Part:BBa_J23100

References