Composite
Part:BBa_K4354013:Design
Designed by: Java Yao Group: iGEM22_GXU-China (2022-07-29)
CcaS/CcaR green-light regulating system in Bacillus subtilis
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1201
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2580
Illegal PstI site found at 1201 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1211
Illegal BglII site found at 1331
Illegal XhoI site found at 4226 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1201
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1201
Illegal AgeI site found at 3244 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We made some optimization in the basis of existing parts.We add our P43 promoter on it and optimize the sequence of ho1 and phyA. The CcaS/CcaR green-light regulating system built by us could realize adjustable regulating for target gene in Bacillus subtilis.
Source
We synthesize it according its sequence and test the accuracy of its sequence.
References
1.Yuu Hirose, Takashi Shimada, Rei Narikawa et al. Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. PNAS. July 15,2008 vol. 105 no.28 9529.