Plasmid
Part:BBa_K4354010:Design
Designed by: Java Yao Group: iGEM22_GXU-China (2022-07-29)
plasmid pHY-P43
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 333
Illegal PstI site found at 311
Illegal PstI site found at 4126 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 333
Illegal PstI site found at 311
Illegal PstI site found at 4126 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 333
Illegal BglII site found at 307
Illegal BamHI site found at 323 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 333
Illegal PstI site found at 311
Illegal PstI site found at 4126 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 333
Illegal PstI site found at 311
Illegal PstI site found at 4126
Illegal AgeI site found at 5005 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2883
Illegal BsaI site found at 4300
Design Notes
We plan to remove the Ampicillin resistance gene because of the incompatibility between plasmid pHY-P43 and pUC19.
Source
We bought it from HonorGene company.