Composite

Part:BBa_K4354009:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-28)


BioBrick used to express EL222


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 898
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 468
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We choose the vector pUC19 to express it because pUC19 has the strong stability in Bacillus subtilis and it is a high copy plasmid, which could express more EL222 protein.


Source

We synthesize it according to its sequence.

References