Coding
NAR
Part:BBa_K4354006:Design
Designed by: Java Yao Group: iGEM22_GXU-China (2022-07-22)
assimilatory nitrate reductase [Dunaliella tertiolecta]
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 367
Illegal PstI site found at 1523
Illegal PstI site found at 1529
Illegal PstI site found at 2287 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1503
Illegal PstI site found at 367
Illegal PstI site found at 1523
Illegal PstI site found at 1529
Illegal PstI site found at 2287 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1088
Illegal BamHI site found at 203 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 367
Illegal PstI site found at 1523
Illegal PstI site found at 1529
Illegal PstI site found at 2287 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 367
Illegal PstI site found at 1523
Illegal PstI site found at 1529
Illegal PstI site found at 2287 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 212
Illegal SapI.rc site found at 355
Design Notes
By cloning AMO,HAO,NAR,NAR,NOR,NOS to the Bacillus subtilis, We want to make it an engineering strain that could remove the nitrogenous compounds in the waste water.
Source
Our team search its sequence from NCBI(AAL79356) and synthesize it.It coms from Dunaliella tertiolecta.
References
1.Song Qin, Xu Lei.Advancement of Enzymology of Heterotrophic Nitrification.BIOTECHNOLOGY BULLETIN.DOI:10.13560/j.cnki.biotech.bull.1985.2008.05.009 2.Xu Tao.METABOLIC CHARACTERISTICS OF HETEROTROPHIC NITRIFYING-AEROBIC DENITRIFYING STRAIN DIAPHOROBACTER SP.PD-7.Taiyuan University of Technology in China.2015 May10.