Coding
AMO

Part:BBa_K4354004:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-22)


ammonia monooxygenase, partial


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 422
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 422
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 422
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 422
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 422
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We clone AMO,HAO,NAR,NIR,NOR,NOS to Bacillus subtilis to make it removal all kinds of nitrogenous compounds.


Source

We search it from NCBI(BAC10331) and synthesize it according to the sequence.

References

1.Song Qin, Xu Lei.Advancement of Enzymology of Heterotrophic Nitrification.BIOTECHNOLOGY BULLETIN.DOI:10.13560/j.cnki.biotech.bull.1985.2008.05.009 2.Xu Tao.METABOLIC CHARACTERISTICS OF HETEROTROPHIC NITRIFYING-AEROBIC DENITRIFYING STRAIN DIAPHOROBACTER SP.PD-7.Taiyuan University of Technology in China.2015 May10.