Composite

Part:BBa_K4350831:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


psLuz Transcriptional Unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 90
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 737
    Illegal PstI site found at 90
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 90
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This transcriptional unit was assembled through golden gate assembly, and all parts required flanking by the appropriate Type IIS restriction and fusion sites.


Source

The sequence for the enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115. The promoter and terminator were acquired from https://doi.org/10.1104/pp.18.01401

References