Composite

Part:BBa_K4350826:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


nnH3H Transcriptional Unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1271
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This transcriptional unit was assembled through golden gate assembly, and all parts required flanking by the appropriate Type IIS restriction and fusion sites.


Source

The sequence for the enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115. The promoter and terminator were acquired from https://doi.org/10.1104/pp.18.01401.

References