Composite

Part:BBa_K4350823:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


nnLuz Transcriptional Unit with Pcpc560-Dx2 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1125
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Each component of this transcriptional unit required complementary fusion sites for proper golden gate assembly with the appropriate Type IIS restriction endonucleases.


Source

This transcriptional unit was assembled by UBC-Okanagan iGEM using golden gate cloning, acquiring the luciferase CDS from https://doi.org/10.1073/pnas.1803615115, codon-optimizing it for Synechocystis sp. PCC 6803. The promoter and terminator were both acquired from the CyanoGate Kit in level 0 backbones (https://doi.org/10.1073/pnas.1803615115).

References