Coding

Part:BBa_K4350821:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


P. stipticus Fungal Luciferase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 700
    Illegal PstI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon-optimized for expression in the model cyanobacterial organism, Synechocystis sp. PCC 6803. Additionally, illegal restriction sites have been removed for compatibility with the MoClo golden gate cloning assembly standard.


Source

The sequence for the enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115.

References