Coding

Part:BBa_K4350818:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


N. nambi Hipidin Synthase (nnHispS)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 63
    Illegal SpeI site found at 1194
    Illegal PstI site found at 904
    Illegal PstI site found at 3173
    Illegal PstI site found at 3926
    Illegal PstI site found at 4142
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1194
    Illegal PstI site found at 904
    Illegal PstI site found at 3173
    Illegal PstI site found at 3926
    Illegal PstI site found at 4142
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1944
    Illegal BglII site found at 4917
    Illegal BamHI site found at 1380
    Illegal BamHI site found at 2089
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 63
    Illegal SpeI site found at 1194
    Illegal PstI site found at 904
    Illegal PstI site found at 3173
    Illegal PstI site found at 3926
    Illegal PstI site found at 4142
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 63
    Illegal SpeI site found at 1194
    Illegal PstI site found at 904
    Illegal PstI site found at 3173
    Illegal PstI site found at 3926
    Illegal PstI site found at 4142
    Illegal NgoMIV site found at 3097
    Illegal AgeI site found at 1295
    Illegal AgeI site found at 3752
    Illegal AgeI site found at 4435
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon-optimized for expression in the model cyanobacterial organism, Synechocystis sp. PCC 6803. Additionally, illegal restriction sites have been removed for compatibility with the MoClo golden gate cloning assembly standard.


Source

The sequence for this enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115.

References