Coding

Part:BBa_K4350817:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


A. nidulans 4′-Phosphopantetheinyl Transferase (NpgA)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 218
    Illegal XhoI site found at 709
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is codon-optimized for expression in the model cyanobacterial organism, Synechocystis sp. PCC 6803. Additionally, illegal restriction sites have been removed for compatibility with the MoClo golden gate cloning assembly standard.


Source

The sequence for this enzyme was retrieved from https://www.pnas.org/doi/10.1073/pnas.1803615115.

References