Terminator

Part:BBa_K4350801:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


Tcpc Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Utilized for the creation of transcriptional units as level 1 parts through assembly with a promoter, RBS, and CDS in a level 1 vector by digesting with Type IIS restriction endonucleases.


Source

Found in the cpc operon of Synechocystis sp. PCC 6803. Available in a level 0 vector compatible with MoClo standard golden gate assembly from the CyanoGate Kit (https://doi.org/10.1104/pp.18.01401).

References