Coding

Part:BBa_K4349001:Design

Designed by: Vanessa Pasa   Group: iGEM22_UFMG_UFV_Brazil   (2022-08-02)


CfcI exochitinase from Aspergillus niger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 518
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 105


Design Notes

The team UFMG_UFV_Brazil tested if the sequence obtained from the literature had any restriction sites that would compromise BioBrick Assembly, and we found one PstI site. We optimized the problematic codon and changed its sequence to maintain the expression of the aminoacid cysteine.

Source

https://www.ncbi.nlm.nih.gov/nuccore/XM_001400452.2?report=fasta

References

van Munster, J. M., van der Kaaij, R. M., Dijkhuizen, L., & van der Maarel, M. J. E. C. (2012). Biochemical characterization of Aspergillus niger CfcI, a glycoside hydrolase family 18 chitinase that releases monomers during substrate hydrolysis. Microbiology, 158(8), 2168–2179. https://doi.org/10.1099/mic.0.054650-0

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