Coding

Part:BBa_K4349000:Design

Designed by: Vanessa Pasa   Group: iGEM22_UFMG_UFV_Brazil   (2022-08-02)


PCCHI44 endochitinase from Pochonia chlamydosporia


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The UFMG_UFV_Brazil team tested the sequence available in the NCBI in the Expasy Translate software and discovered that the original sequence still had 5'UTR and 3'UTR nucleotides. After that, we removed the sequences that were not translated as our protein of interest, resulting in the DNA sequence posted here. We also tested if the resulting sequence had any restriction sites that would compromise BioBrick Assembly, but there were not.

Source

https://www.ncbi.nlm.nih.gov/nuccore/EU128676.1?report=fasta

References

Mi, Q., Yang, J., Ye, F., Gan, Z., Wu, C., Niu, X., Zou, C., & Zhang, K.-Q. (2010). Cloning and overexpression of Pochonia chlamydosporia chitinase gene pcchi44, a potential virulence factor in infection against nematodes. Process Biochemistry, 45(5), 810–814.