Coding

Part:BBa_K4343109

Designed by: Yue Shu   Group: iGEM22_NNU-China   (2022-10-09)


MaElo16

MaElo16 encodes C16/18 elongase from Misgurnus anguillicaudatus which functions in the desaturase and elongase pathway and could convert C16:0 to C18:0.

Description

The wild-type Y. lipolytica Po1f can produce C18:0 under the catalysis of C16/18 elongase. MaElo16 encodes C16/18 elongase of 266 amino acids from Misgurnus anguillicaudatus. Heterologous expression of MaElo16 could catalyze C16:0 to C18:0 in Y. lipolytica. 

Some studies have used C16/18 elongase from this source to produce high yields of omega-3 fatty acids.

Reference

Chen, J., Cui, Y., Yan, J., Jiang, J., Cao, X., & Gao, J. (2018). -A>. Gene, 666, 134-144.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 571
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 818


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Parameters
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