Coding

Part:BBa_K4343107

Designed by: Xiner Liu   Group: iGEM22_NNU-China   (2022-10-09)


PsElo9

Heterologous expression of PsElo9 could enable engineered Y. lipolytica to produce C20:2 fatty acids that couldn’t be produced in wild-type Y. lipolytica.

Characterization

Heterologous expression of PsElo9 could enable engineered Y. lipolytica to produce C20:2 fatty acids that couldn’t be produced in wild-type Y. lipolytica.

Literature

Petrie, JR completed the isolation of the entire P. salina docosahexaenoic acid (DHA) pathway through the identification of the Pavlova salina (N. Carter) J. C. Green Delta 9-elongase, and described the assembly of this pathway in Nicotiana benthamiana.

Reference

Petrie, J. R., Mackenzie, A. M., Shrestha, P., Liu, Q., Frampton, D. F., Robert, S. S., & Singh, S. P. (2010). ISOLATION OF THREE NOVEL LONG‐CHAIN POLYUNSATURATED FATTY ACID Δ9‐ELONGASES AND THE TRANSGENIC ASSEMBLY OF THE ENTIRE PAVLOVA SALINA DOCOSAHEXAENOIC ACID PATHWAY IN NICOTIANA BENTHAMIANA 1. Journal of Phycology, 46(5), 917-925.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 142
    Illegal BsaI.rc site found at 857


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Categories
Parameters
None