Part:BBa_K4343104

IgASE1

IgASE1 encodes ∆-9 elongase from Isochrysis galbana which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Characterization

Heterologous expression of IgASE1 could enable engineered Y. lipolytica to produce C20:2 fatty acids that couldn’t be produced in wild-type Y. lipolytica.

Literature

Baoxiu Qi characterized the function, activity and substrate specificity of IgASE1 in Saccharomyces cerevisiae. The engineered yeast cells could specifically convert the C18-∆9 polyunsaturated fatty acids, linoleic acid (C18:2n-6, ∆9;12) and α-linolenic acid (C18:3n-3, ∆9;12;15), to eicosadienoic acid (C20:2n-6, ∆11;14) and eicosatrienoic acid (C20:3n-3, ∆11;14;17), respectively.

Reference

Qi, B., Beaudoin, F., Fraser, T., Stobart, A. K., Napier, J. A., & Lazarus, C. M. (2002). Identification of a cDNA encoding a novel C18-Δ9 polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana. FEBS letters, 510(3), 159-165.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 246
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 809
  Illegal SapI site found at 290
  Illegal SapI.rc site found at 773