Coding

Part:BBa_K4343095

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


EgElo9

EgElo9 encodes ∆-9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Characterization

The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6).

Reference

1. Zhu, Q. 365, 307–316 (1999). et al. Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. in Single Cell Oils: Microbial and Algal Oils (eds. Cohen, Z. 12. & Ratledge, C.) 51–73 (AOCS press, Urbana, 2010).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 354
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794


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